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Bio-Techne
United States
Приєднався 29 жов 2012
Bio-Techne is a leading supplier of high quality and innovative tools for Life Science Research, Therapeutic Manufacturing and Clinical Diagnostics.
www.bio-techne.com
www.bio-techne.com
Witness The Revolution: Introducing Leo, the Next Leap in Simple Western Technology
www.bio-techne.com/leo
The Leo™ System, powered by Simple Western™ Technology, is a high-throughput, fully automated capillary western platform. It enables fast, simple, and sensitive protein quantification and detection, processing up to 100 samples per run, increasing experimental size for quantitative assay designs and delivering results in as little as 3 hours.
The Leo™ System, powered by Simple Western™ Technology, is a high-throughput, fully automated capillary western platform. It enables fast, simple, and sensitive protein quantification and detection, processing up to 100 samples per run, increasing experimental size for quantitative assay designs and delivering results in as little as 3 hours.
Переглядів: 240
Відео
Move Beyond Traditional Western Blots with Leo
Переглядів 41114 днів тому
www.bio-techne.com/leo The Leo™ System, powered by Simple Western™ Technology, is a high-throughput, fully automated capillary western platform. It enables fast, simple, and sensitive protein quantification and detection, processing up to 100 samples per run, increasing experimental size for quantitative assay designs and delivering results in as little as 3 hours.
Analytical Exchange 2023 Overview
Переглядів 44Місяць тому
Pre-register for Analytical Exchange 2025, our highly anticipated annual European Instruments User Meeting-an immersive two-day event that unites the vibrant community of users of our cutting-edge technologies. www.bio-techne.com/about/events/european-instruments-user-meeting
School of RNAscope 2024 Overview
Переглядів 3275 місяців тому
School of RNAscope 2024 - Connect and Learn About the Latest Innovative RNAscope ISH Technology Applications. Join us for this 2-day event, bringing together the widest range of RNAscope users, to advance your spatial biology insights. 13th and 14th May 2024 Edgbaston Park Hotel & Conference Centre, Birmingham, UK Register here: www.bio-techne.com/about/events/schoolofrnascope
Introducing Back of the Napkin - Inspiring Stories from Biotech Pioneers
Переглядів 350Рік тому
A discovery is said to be an accident meeting a prepared mind, but every story behind a discovery is different. Perhaps the idea is conceived in a light bulb moment or brainstorming session or captured in scribblings on the back of a napkin. All these great game-changing discoveries seem to have one thing in common: the pioneers, the scientists and the entrepreneurs that conceive the ideas aren...
Maurice: One Instrument for CE-SDS and cIEF
Переглядів 840Рік тому
Learn more about Maurice: www.bio-techne.com/Maurice Maurice is your one-stop instrument for automated cIEF and CE-SDS. Get protein charge and size data on a variety of biotherapeutics-mAbs, ADCs, fusion proteins, vaccines, and more. Just pop in one of the ready-to-go cartridges, drop in your sample vials or a 96-well plate, and hit start. Switch between cIEF and CE-SDS applications as often as...
Workshop: Total Protein and Protein Normalization with Simple Western™
Переглядів 1,9 тис.Рік тому
More information on Simple Western™: www.bio-techne.com/instruments/simple-western Assay development for qualitative, semi-quantitative, or quantitative analysis. Discussion of antibody saturation and linear dynamic range. More Workshops: academy.bio-techne.com/learn
Workshop: Total Protein and Protein Normalization
Переглядів 1,7 тис.Рік тому
This is a workshop introduction to Simple Western™: www.bio-techne.com/instruments/simple-western Learn about assay development for qualitative, semi-quantitative, or quantitative analysis. We’ll also discuss antibody saturation and linear dynamic range. For more workshops go to academy.bio-techne.com/learn
Workshop: Bioassay Development on Simple Western™
Переглядів 924Рік тому
Simple Western™ assays for success: www.bio-techne.com/instruments/simple-western How to select the right antibody; potency assays. For more workshops go to academy.bio-techne.com/learn More training: academy.bio-techne.com/learn
Fundamentals: See What Happens Inside of a Charge Capillary
Переглядів 178Рік тому
This video will show you what happens inside of a charge capillary within the Peggy Sue instrument: www.bio-techne.com/instruments/simple-western/simple-western-assays-charge-based-separation This can also apply to cIEF for Maurice. We’ll discuss ampholytes, PI, etc. For more training, go to academy.bio-techne.com/learn Peggy Sue: bio-techne.com/p/simple-western/peggy-sue_004-800
How to Set-Up Wes Training Kits
Переглядів 368Рік тому
This video will show you how to use the Wes Training Kit. Wes: www.bio-techne.com/p/simple-western/wes_004-600 For more training go to the Wes Learning Plan in the Bio-techne Academy. academy.bio-techne.com/learn
How to set up a Maurice capillary isoelectric focusing (cIEF) assay
Переглядів 2,5 тис.Рік тому
This video will show you how to run a protein charge assay using imaged capillary isoelectric focusing (icIEF) cartridges on CE platform Maurice and Maurice C. Real time whole column imaging and peak resolution allows rapid precipitation or aggregation issue identification in protein samples. Learn more about Maurice here -www.bio-techne.com/Maurice. This video is part of a series of trainings ...
How to Set-Up Jess Training Kits
Переглядів 376Рік тому
This video will show you how to use the Jess Training Kit. More info on Jess: www.bio-techne.com/p/simple-western/jess_004-650 For complete Jess training visit the Bio-techne Academy: academy.bio-techne.com/learn
What You Need to Know About the Peggy Sue Instrument
Переглядів 247Рік тому
This video will introduce you the basics of the Peggy Sue Instrument: bio-techne.com/p/simple-western/peggy-sue_004-800 For more Peggy Sue Training go to the Peggy Sue Learning Plan in the Bio-Techne Academy: academy.bio-techne.com/learn
How to prepare your Jess Plate with Stellar Detection
Переглядів 349Рік тому
This video will show you how to use Stellar with a Jess Instrument. For more stellar or Jess training go to the Jess Learning Plan in the Bio-techne Academy. Academy.bio-techne.com
Meet Jess: an Automated Western Blot System
Переглядів 7 тис.Рік тому
Meet Jess: an Automated Western Blot System
Meet Sally Sue: an Automated Protein Size Based Separation System
Переглядів 422Рік тому
Meet Sally Sue: an Automated Protein Size Based Separation System
Introducing the Turbo CE-SDS Cartridge for Maurice
Переглядів 763Рік тому
Introducing the Turbo CE-SDS Cartridge for Maurice
Ella Instrument: Precision Multiplexing Made Simple
Переглядів 6 тис.Рік тому
Ella Instrument: Precision Multiplexing Made Simple
MauriceFlex: icIEF, CE-SDS, and icIEF Fractionation
Переглядів 2,9 тис.Рік тому
MauriceFlex: icIEF, CE-SDS, and icIEF Fractionation
Join Us at Our Next European Instrument User Meeting
Переглядів 191Рік тому
Join Us at Our Next European Instrument User Meeting
Meet the Analytical Solutions Division Team
Переглядів 149Рік тому
Meet the Analytical Solutions Division Team
Bio-Techne Molecular Controls - Armored RNA Quant®
Переглядів 251Рік тому
Bio-Techne Molecular Controls - Armored RNA Quant®
Bringing Together Our Instrument Users at Our European Instrument User Meeting
Переглядів 79Рік тому
Bringing Together Our Instrument Users at Our European Instrument User Meeting
Bio-Techne - Together Transforming Companion Diagnostics
Переглядів 514Рік тому
Bio-Techne - Together Transforming Companion Diagnostics
Turbo CE-SDS by ProteinSimple, a Bio-Techne Brand
Переглядів 779Рік тому
Turbo CE-SDS by ProteinSimple, a Bio-Techne Brand
Very useful video. Can you tell us what software you used to analyze the data? There has been no answer to the previous question. Thanks for your attention.
Good I don’t have to wonder if I’m getting hiv or drugged never know whats in your goodie bag
hello :) is it possible to damage the antibodies by vortexing them after dilution ?
how long can i storage the antibody-coated elisa plate in the refrigerator?
R&D Systems has validated the DuoSet plate preparation method as written in the product datasheet - plates are coated overnight and assayed the next day. Longer incubation of the Capture Antibody plate coating (step 1 of the Plate Preparation) is not recommended. For short-term (24 hour storage), stop at the blocking step (Step 3 of the Plate Preparation Protocol) and store the plate wet in the Reagent Diluent blocking buffer overnight at 4 degrees Celsius. Equilibrate the plate to room temperature the next day and proceed with assay protocol. Blocking longer than 24 hours is not recommended. For more FAQs please see our ELISA resource page: www.rndsystems.com/resources/faqs/elisas
what's the difference between raw material and starting material? Are starting material considered as one kind of raw material, but just more critical?
cost?
Hello - please fill this request form to receive information on pricing for Jess: www.bio-techne.com/p/instruments/simple-western/request-quote
Whomuch instrument cost test Manu
Is it possible to aliquot the reconstituted standard and store it at -80°C? If so, for how long?
It is generally not recommended to aliquot and store reconstituted standards. The suitability for long-term storage varies depending on the analyte. Most standards are intended for single-use only to ensure accurate results. It's best to follow the guidelines specific to each analyte. If you have further questions please contact us: www.bio-techne.com/support/contact-us
Saved my morning, thank you
How long does it take to do the entire workflow?
Hello mam you have provide stem cell therapy Neurogenic bladder
Is it mandatory to use controls with each run?
It is not mandatory, but we recommend testing controls in each assay for evaluating performance. Please refer to your kit booklet for more information or contact our support team: www.bio-techne.com/support/contact-us
Wonderful talk!!!
thank you!!
What is an IP?
5:40
Hi. Really nice and useful video. Can you please tell which software you used for data analysis? Thank you.
Great lecture
wow, chymotrypsin-like cleavage (beta5) might be most important for longevity 34:37 "key lysine residue" at 27:00?
very helpful
How does this get into the cell?
Thanks for this great lecture! It includes so many insights, facts and pitfalls that are so easy to overlook.
thank you!
Very nice video. Sorry to ask, is it necessary to have the handheld magnetic washer?
Yes! The magnetics attach the beads to the base of the well so they are not lost when discarding the solutions. Without the magnetic washer the microbeads will go down the sink too and you'll be left with nothing to analyse! Make sure you allow 1 min for the beads to settle before discarding solutions too.
9:30 start of presentation 13:20 ubiquitin-proteasome pathway summary slide - ATP required 15:22 *antigen presentation* - 1% of product peptides are taken up by ER and displayed in cell surface by MHC class I molecules - proteasome allows immune system to screen intracellular space for abnormal proteins (pathogen or cancer) 23:16 multiple ATP dependent steps in proteasome function 38:00 Bortezmib - proteasome inhibitor, inhibits NF-kB, does not cross BBB, induces apoptosis 40:33 Endoplasmic-reticulum-associated protein degradation () pathway is unuslaully activated in multiple myeloma cells ; proteasome inhibition results in increased ER stress and triggers UPR, further UPR activation triggers apoptosis 42:30 proteasome inhibitors are not more toxic since they mainly block chymotrypsin-like site & only partially inhibit protein degradation ; since myeloma cells have to break down lots of immunoglobins they become more stressed than other cells as a result 43:27 cells can compensate for proteasome inhibition thru Nrf1 activation (more proteasome) and increase in autophagy / lysosomal degradation 44:35 cAMP phosphorylation of 26S proteasome enhances degradation of misfolded proteins 45:50 cAMP dependent regulation of protein phosphorylation ; rolipram is PDE4 inhibitor 47:39 cAMP activation for treatment of neurodegenerative diseases 49:06 rolipram for taupathology ; rolipram increased proteasome activity and reduced tau accumulation in mouse model 50:36 summary slide - endogenous regulation (ubiquitination, DUBs) ; exogenous activation - phosphorylation of proteasome 52:13 Q&A
very good video
Thank u !! I have an exam tomorrow of this and It helped me a lot
What is the purpose of phosphate buffered saline?
it mimics physiologic pH and ion concentration
thanks for this video
But what isn't addressed here and most commonly anywhere else is how long do we need to fast and at whicj juncture - is it at 16 hrs without food or 24 or 36? Then how long do we stay without food for this to be optimally beneficial. Tell.us the whole story. Break it down to a simplistic practical application please.
I’ve seen in other studies that a3 day fast is optimal
On your investor web page you titled this video "Epic Tools for Epic Science" (investors.bio-techne.com/). That is a lot more compelling than "A Reputation of Quality: The Bio-Techne Brand Story". Own it.
Hey. Excellent webinar!!. I have one question, in normoxia, how many passages can we culture the MSCs?.. Also, we are facing difficulty in trypsinization of MSCs. any suggestions??
TrypLE
Hi S.Sankara, sorry for the delay in our reply. Please see the response from Marnelle Anderson, one of our research associates. "If you are growing cells in high FBS, then sometimes you may need to rinse several times with PBS, or perhaps to quickly rinse with trypsin prior to trypsinization. If you are growing them in serum free media, they should come off easily. The caveat is that MSCs get stickier when they start to differentiate. As to how many passages you can expect out of primary MSCs, this is trickier, since experience has shown it depends on the quality of the MSC, where they are isolated from, and the media. In general, I would say 5-10 passages… five being a decent/ average number and 10 being a great one. " Please let us know if we can answer any additional questions.
I love your slides. Very comprehensable explanations too 5|5
Does this work with all cells lines?
Nice video
Good work!
23:00 ,2nd lecturE obecity impact on stomach & cancer
Is it in the competition with Organs on Chip technology ...this is more accurate and has more reliable results ...
I am not from the scientific community. However I have been practicing “ starvation” to activate autophagy the last 10 years without realizing it. I take just 1 meal daily. Dinner. I skip breakfast and lunch. I do not eat anything after dinner. No special diet. What is most interesting to me is the realization that the ancients have knew about autophagy all this while. Muslims fast. The gurus of India and the lama in Tibet and Nepal fast on a regular basis. They know the benefits of autophagy. The only difference is the do not understand mechanism of it until 20th/21st century with the ground breaking discovery of Dr Yoshinori Ohsumi. It is also very interesting to find out that certain existing drugs may help in activating autophagy. Metformin for example has is commonly prescribed for diabetes.
May be the reason why when we r not felling well we don't have the appetite to eat and just sleep n rest until we r well may be part of how our bodies heal themselves? Hmmm...
look up dry fasting for the true fast ;)@@vister6757
Go to 3:41 to skip the tedious introduction
You can say that again... damn
So people with ms should avoid dairy?
thank you for uploading this, I am beginning my honours project this year which is related to ARMeD destruction of endogenous proteins. this video on SUMO has helped me get my head around the basics.